Table 12 Capillaries tab—sequencing install run (continued)
Result Description
Alignment read length Length and range of the sample sequence in which basecalling accuracy is
>99% relative to the known reference sequence of the sequencing install
standard. It does not consider QV. It does consider the entire sequence.
The Alignment read length value is derived from basecall accuracy (% RL
basecall), and not from quality values.
% RL basecall
(This result is referred to
as Accuracy in the plot
view.)
The percent of basecalling accuracy within the alignment read length and range.
Install QC
(This result is referred to
as QC on the info/details
screen.)
•
—All QC tests passed.
•
—At least 1 warning quality alert was triggered.
•
—At least 1 failing quality alert was triggered.
For more information, see “Sample QC and quality alerts” on page 172
Details screen—sequencing install run
Chapter 12 Run calibrations and install checks
Perform an install run
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SeqStudio
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Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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