The red line in the plot is the sizing curve. The install run uses the Global Southern sizing method.
Y
ou can tap View by to filter results by Pass (
) or Flagged (
or ).
F
or more information, see “Use the electropherogram screen” on page 173.
Pass/fail criteria and results for the fragment install check
The software evaluates peaks in the data for each capillary. To be identified as a possible allele, peaks
must be within the following ranges. The nominal allele size, or reference bin size, is hard-coded in the
software.
Result Acceptance criteria
# Allele peaks 15 per capillary
# Size standard peaks 34 per capillary
Sizing precision (standard deviation of the observed allele fragment
siz
es for all capillaries)
<0.15 base pairs (bp)
For all peaks that meet the acceptance criteria and are within the nominal size range, the software
calculat
es the Average Peak Height and the Sizing Precision. Peaks that meet the following
thresholds pass.
The following information is included in an exported install report.
Chapter 12 Run calibr
ations and install checks
Perform an install run
12
350
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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