Table 17 Sequencing analysis run modules (continued)
Run module
type
Run module name
Configuration 23 hours throughput
[1]
Perform
- ance
Cap.
length
(cm)
Polymer
type
Run
time
(min)
(8‑cap.) (24-cap.)
Contig.
Read
Length
(CRL)
[2]
Standard
sequencing
BigDye
XTerminator
™
BDxStdSeq50_POP6
BDxStdSeq50_POP6xl
50 POP-6
™
≤140 ≥72 ≥216 ≥600
BDxStdSeq50_POP7
BDxStdSeq50_POP7xl
50 POP-7
™
≤125 ≥88 ≥264 ≥850
Microbial
sequencing
for
MicroSEQ
™
ID kits
MicroSeqID50_POP6
MicroSeqID50_POP6xl
50 POP-6
™
≤135 ≥80 ≥240 ≥600
[1]
T
hroughput (samples/day): The total number of samples run in 23 hours (0.5 hour is required for user interaction and 0.5 hour is required for
warm-up time).
[2]
The maximum number of contiguous bases in the analyzed sequence with an average QV ≥20, calculated over a sliding window 20 base pairs
wide from an AB Long Read Standard sequencing sample. This calculation starts with base number 1. The read length is counted from the
middle base of the first good window to the middle base of the last good window, where a “good” window is one in which the average QV is
≥20.
Appendix B Run modules and dye sets
Run modules, r
ead lengths, size ranges, and run times
B
528
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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