Thermo Scientific appliedbiosystems SeqStudio Flex Series - Page 569

regular run 146
precautions for use 17
preheat the oven and detection cell 68
primer peak, eliminating 295
print plate layout, Plate Manager 125
prioritize an injection in the injection list 46, 163
probability of error, QV 178
profile
change local profile from standard to administra‐
tor 447
create local 29, 55, 448
delete local or cloud from instrument 447
local and cloud, comparison 213
local, administrator and standard 446
local, edit 447
options 29, 55
PIN, change 451
user initials displayed in software 213
when to use local vs cloud 213
proximity sensor, self test 485
PS software. See Peak Scanner software
PSM file
contents 86, 98, 110
create or open in Plate Manager 115
download, Plate Manager 129
export or delete 264
when to use 86, 98, 110
pump
avoid damage 377
bubbles, remove 377
check for bubbles or debris 480
flush water trap 379
maintenance 376
maintenance wizard 415
parts of 21, 376, 487
trap, flush 379
wash chamber and channels 379
water trap, flush 379
push plate file. See send plate file
Q
QC, sample 172
QC error and alert troubleshooting 489
QC errors
fragment analysis 489, 490
sequencing 489, 490
sequencing install run 494
spectral calibration (manual) 492
QC report 171, 469
QC software. See Quality Check software
quality alerts
basecalling 489, 490
fragment analysis 490
sequencing 490
sequencing install run) 494
sizecalling 490
spectral calibration (manual) 492
Quality Check software, results, download 207
quality flag 180
quality tests, data 172
quality value
See also QV
pure base vs mixed base 178
spectral calibration 320
See also QV
QV, probability of error 178
QV range
bar color 178
bar height 178
values 178
QV20 CRL, defined 176
QV20 CRL and range for sequencing standards 346
QV20 CRL basecall 341, 344
R
raw data 476
re-injection specify
plate file 92
Remote Monitoring software 192
Remote Monitoring software, re-injection com‐
mand not sent to instrument 192
Run queue 165
reactivate the instrument, maintenance wizard 427
related documentation 473, 560
release notes for software update, export from in‐
strument 462
Remote Monitoring software
See also cloud analysis
alarm clock icon 184
cancel command not sent to instrument 192
cloud analysis, set up 205
open from InstrumentConnect 183
open from Plate Manager (cloud 182
parts of 184
pause instrument 193
PNG, save 190
re-inject 192
re-inject command not sent to instrument 192
requirements for viewing results 187
results, download 201
results, export 201
results, view cloud analysis 207
results, view completed (Run history) 198
results, view real-time 190
spectral calibration or install run results are not
displayed in Run history tab 202
Index
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
569